2017-8-11 16:0:32025-05-31 03:04:46
_ec
description
ATP-dependent dsDNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum
ATP-dependent dsDNA translocase, resolution of chromosomal dimers after [SW|DNA replication], transports the forespore chromosome across the [SW|sporulation] septum
locus
BSU16800
BSU_16800
proteinLength
787
789
geneLength
2361
2370
function
chromosome partition during sporulation
chromosome partition during [SW|sporulation]
outlinks
bsu
BSU16800
BSU_16800
Gene
Coordinates
1,752,272 → 1,754,641
1,752,272 1,754,641
The protein
Catalyzed reaction/ biological activity
transports the forespore chromosome across the sporulation septum [Pubmed|20516200]
translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes [Pubmed|19818024]
the two DNA translocases [[protein|SftA]] and [[protein|SpoIIIE]] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG [Pubmed|18391964]
transports the forespore chromosome across the [SW|sporulation ]septum [Pubmed|20516200]
translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes [Pubmed|19818024]
the two DNA translocases [[protein|SftA]] and [[protein|SpoIIIE]] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG [Pubmed|18391964]
The protein
Paralogous protein(s)
[[protein|SftA]]
[[this]]
The protein
[SW|Domains]
N-terminal transmembrane domain (4 helices) [Pubmed|24297254]
134 aa linker [Pubmed|24297254]
512 aa motor domain at the C-terminus [Pubmed|24297254]
N-terminal transmembrane domain (4 helices) [Pubmed|24297254]
134 aa linker [Pubmed|24297254]
512 aa motor domain at the C-terminus [Pubmed|24297254]
[SW|FtsK domain] (aa 450-646) (according to UniProt)
The protein
[SW|Localization]
transmembrane domain mediates dynamic localization to active division sites [Pubmed|20516200]
complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of [SW|sporulation] and division septa [Pubmed|23667326]
free diffusion in the membrane, no enrichment at septa [pubmed|29439991]
transmembrane domain mediates dynamic localization to active division sites [Pubmed|20516200]
complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of [SW|sporulation] and division septa [Pubmed|23667326]
Biological materials
Mutant
BKE16800 (''spoIIIE''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center] [pubmed|28189581]
BKE16800 ([[gene|spoIIIE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
BKK16800 ([[gene|spoIIIE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
References
Reviews
References
Original publications
The protein
Protein family
FtsK/SpoIIIE/SftA family (with [[protein|SftA]], according to UniProt)